Journal: Antioxidants
Article Title: Mitochondrial Abnormalities in Induced Pluripotent Stem Cells-Derived Motor Neurons from Patients with Riboflavin Transporter Deficiency
doi: 10.3390/antiox9121252
Figure Lengend Snippet: Confocal analysis of riboflavin transporter deficiency (RTD) vs. Ctrl motor neurons (MNs). ( a – d ) Immunofluorescence images showing the distribution of βIII-Tubulin (βIII-TUB) (red) and superoxide dismutase 2 (SOD2) (green). Nuclei are stained with Hoechst (blue). White arrows indicate neurites in ( a , c – e ) Significantly lower levels of βIII-TUB and of the mitochondrial marker SOD2 are detected in diseased MNs (* p ≤ 0.05 vs. Ctrl MNs; ** p ≤ 0.01 vs. Ctrl MNs). White columns indicate Ctrl cells, while grey ones indicate diseased MNs.
Article Snippet: For confocal analysis, cells underwent our previously described double immunofluorescence protocol [ ], using the following primary antibodies: (i) Rabbit polyclonal antibody against mitochondrial superoxide dismutase 2 (anti-SOD2, ab13533, Abcam); (ii) mouse monoclonal antibody to βIII-Tubulin (T8578, Sigma-Aldrich); and (iii) rabbit polyclonal antibody against Neurofilament 200 (or SMI-32, N4142, Sigma-Aldrich).
Techniques: Immunofluorescence, Staining, Marker