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Biospes Inc polyclonal anti-superoxide dismutase 2
Polyclonal Anti Superoxide Dismutase 2, supplied by Biospes Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal anti-superoxide dismutase 2/product/Biospes Inc
Average 90 stars, based on 1 article reviews
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Confocal analysis of riboflavin transporter deficiency (RTD) vs. Ctrl motor neurons (MNs). ( a – d ) Immunofluorescence images showing the distribution of βIII-Tubulin (βIII-TUB) (red) and superoxide <t>dismutase</t> <t>2</t> <t>(SOD2)</t> (green). Nuclei are stained with Hoechst (blue). White arrows indicate neurites in ( a , c – e ) Significantly lower levels of βIII-TUB and of the mitochondrial marker SOD2 are detected in diseased MNs (* p ≤ 0.05 vs. Ctrl MNs; ** p ≤ 0.01 vs. Ctrl MNs). White columns indicate Ctrl cells, while grey ones indicate diseased MNs.
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Confocal analysis of riboflavin transporter deficiency (RTD) vs. Ctrl motor neurons (MNs). ( a – d ) Immunofluorescence images showing the distribution of βIII-Tubulin (βIII-TUB) (red) and superoxide <t>dismutase</t> <t>2</t> <t>(SOD2)</t> (green). Nuclei are stained with Hoechst (blue). White arrows indicate neurites in ( a , c – e ) Significantly lower levels of βIII-TUB and of the mitochondrial marker SOD2 are detected in diseased MNs (* p ≤ 0.05 vs. Ctrl MNs; ** p ≤ 0.01 vs. Ctrl MNs). White columns indicate Ctrl cells, while grey ones indicate diseased MNs.
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Confocal analysis of riboflavin transporter deficiency (RTD) vs. Ctrl motor neurons (MNs). ( a – d ) Immunofluorescence images showing the distribution of βIII-Tubulin (βIII-TUB) (red) and superoxide <t>dismutase</t> <t>2</t> <t>(SOD2)</t> (green). Nuclei are stained with Hoechst (blue). White arrows indicate neurites in ( a , c – e ) Significantly lower levels of βIII-TUB and of the mitochondrial marker SOD2 are detected in diseased MNs (* p ≤ 0.05 vs. Ctrl MNs; ** p ≤ 0.01 vs. Ctrl MNs). White columns indicate Ctrl cells, while grey ones indicate diseased MNs.
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Confocal analysis of riboflavin transporter deficiency (RTD) vs. Ctrl motor neurons (MNs). ( a – d ) Immunofluorescence images showing the distribution of βIII-Tubulin (βIII-TUB) (red) and superoxide <t>dismutase</t> <t>2</t> <t>(SOD2)</t> (green). Nuclei are stained with Hoechst (blue). White arrows indicate neurites in ( a , c – e ) Significantly lower levels of βIII-TUB and of the mitochondrial marker SOD2 are detected in diseased MNs (* p ≤ 0.05 vs. Ctrl MNs; ** p ≤ 0.01 vs. Ctrl MNs). White columns indicate Ctrl cells, while grey ones indicate diseased MNs.
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Confocal analysis of riboflavin transporter deficiency (RTD) vs. Ctrl motor neurons (MNs). ( a – d ) Immunofluorescence images showing the distribution of βIII-Tubulin (βIII-TUB) (red) and superoxide dismutase 2 (SOD2) (green). Nuclei are stained with Hoechst (blue). White arrows indicate neurites in ( a , c – e ) Significantly lower levels of βIII-TUB and of the mitochondrial marker SOD2 are detected in diseased MNs (* p ≤ 0.05 vs. Ctrl MNs; ** p ≤ 0.01 vs. Ctrl MNs). White columns indicate Ctrl cells, while grey ones indicate diseased MNs.

Journal: Antioxidants

Article Title: Mitochondrial Abnormalities in Induced Pluripotent Stem Cells-Derived Motor Neurons from Patients with Riboflavin Transporter Deficiency

doi: 10.3390/antiox9121252

Figure Lengend Snippet: Confocal analysis of riboflavin transporter deficiency (RTD) vs. Ctrl motor neurons (MNs). ( a – d ) Immunofluorescence images showing the distribution of βIII-Tubulin (βIII-TUB) (red) and superoxide dismutase 2 (SOD2) (green). Nuclei are stained with Hoechst (blue). White arrows indicate neurites in ( a , c – e ) Significantly lower levels of βIII-TUB and of the mitochondrial marker SOD2 are detected in diseased MNs (* p ≤ 0.05 vs. Ctrl MNs; ** p ≤ 0.01 vs. Ctrl MNs). White columns indicate Ctrl cells, while grey ones indicate diseased MNs.

Article Snippet: For confocal analysis, cells underwent our previously described double immunofluorescence protocol [ ], using the following primary antibodies: (i) Rabbit polyclonal antibody against mitochondrial superoxide dismutase 2 (anti-SOD2, ab13533, Abcam); (ii) mouse monoclonal antibody to βIII-Tubulin (T8578, Sigma-Aldrich); and (iii) rabbit polyclonal antibody against Neurofilament 200 (or SMI-32, N4142, Sigma-Aldrich).

Techniques: Immunofluorescence, Staining, Marker